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Resumo O objetivo do presente estudo é avaliar os efeitos das nanopartículas de selênio no crescimento, hematologia e digestibilidade dos nutrientes de alevinos de Labeo rohita. Os alevinos foram alimentados com sete dietas isocalóricas à base de farinha de girassol suplementada com diferentes concentrações de nanopartículas, nomeando T1 a T7 (0, 0,5, 1, 1,5, 2, 2,5 e 3 mg / kg), com 5% do peso corporal úmido enquanto o óxido crômico foi usado como um marcador indigesto. Após a experimentação por 90 dias, o grupo tratado com T3 (nível 1mg / kg -1Se-nano) mostrou o melhor resultado em parâmetros hematológicos (WBC’s 7,97 × 103mm-3, RBC’s 2,98 × 106mm-3 e contagem de plaquetas 67), digestibilidade dos nutrientes (proteína bruta: 74%, extrato de éter: 76%, energia bruta: 70%) e desempenho de crescimento (ganho de peso 13,24 g, ganho de peso % 198, taxa de conversão alimentar 1,5, taxa de sobrevivência 100%) em comparação com os outros grupos de tratamento. As taxas de crescimento específicas foram encontradas significativamente mais altas em T5 do que em outros grupos. O presente estudo indicou efeito positivo de 1 mg / kg de nanopartículas de Se no avanço do crescimento, parâmetros hematológicos e digestibilidade de nutrientes de alevinos de L. rohita.

Abstract The aim of the present study is to assess the effects of selenium nanoparticles on the growth, hematology and nutrients digestibility of Labeorohita fingerlings. Fingerlings were fed with seven isocaloric sunflower meal-based diet supplemented with different concentrations of nanoparticles naming T1 to T7 (0, 0.5, 1, 1.5, 2, 2.5, and 3 mg/kg), with 5% wet body weight while chromic oxide was used as an indigestible marker. After experimentation for 90 days T3 treated group (1mg/kg -1Se-nano level) showed the best result in hematological parameters (WBC’s 7.97 ×103mm-3, RBC’s 2.98 ×106 mm-3 and Platelet count 67), nutrient digestibility (crude protein: 74%, ether extract: 76%, gross energy: 70%) and growth performance (weight gain 13.24 g, weight gain% 198, feed conversion ratio 1.5, survival rate 100%) as compared to the other treatment groups. Specific growth rates were found significantly higher in T5 than in other groups. The present study indicated positive effect of 1 mg/kg Se-nanoparticles on growth advancement, hematological parameters, and nutrients digestibility of L. rohita fingerlings.

https://doi.org/10.1590/1519-6984.253555

Molecular based identification and phylogenetic relationship by using cytochrome b gene of Pangasius pangasius
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Hassan, S.

; Naeem, M.

; Nasir, M. Farhan

; Riaz, P.

; Khan, M. N.

; Atiq, I.

Brazilian Journal of Biology

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2024, Volume 84 elocation e268001

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo A abordagem molecular para identificação de espécies desconhecidas usando o gene citocromo b é eficaz e confiável em comparação com a identificação baseada na morfologia. Códigos de barras de DNA de genes moleculares universais foram usados para identificar as espécies. O citocromo b é um gene específico usado para fins de identificação. O código de barras de DNA é um método confiável e eficaz em comparação com os diferentes métodos morfológicos tradicionais de identificação de espécies. Assim, no presente estudo, que foi realizado para identificar as espécies, um total de 50 amostras de peixes foram coletadas em cinco locais diferentes. O DNA foi extraído usando o método Fenol Clorofórmio do tecido muscular. Cinco sequências foram sequenciadas (uma de cada local), analisadas e identificadas espécies específicas, como Pangasius pangasius. As sequências identificadas tinham comprimento variável de 369 bp (Local 1), 364 bp (Local 2), 369 bp (Local 1), 364 bp (Local 3), 352 bp (Local 4) e 334 bp (Local 5). As correspondências de identidade no banco de dados do NCBI confirmaram a espécie específica como P. pangasius. Uma árvore de distanciamento foi desenhada para mostrar a máxima probabilidade entre elas e diferentes espécies. No entanto, em muitos casos, peixes em diversos estágios de desenvolvimento são difíceis de identificar por caracteres morfológicos. Os métodos de identificação baseados em DNA oferecem uma adição analiticamente poderosa ou mesmo uma ferramenta alternativa para identificação de espécies e estudo filogenético. Este trabalho pretende fornecer uma visão geral atualizada e abrangente sobre os métodos baseados em DNA para identificação de espécies de peixes usando o gene citocromo b como marcadores direcionados para fins de identificação.

Abstract Molecular appraoch for identification of unknown species by using Cytochrome b gene is an effective and reliable as compared with morphological based identification. For DNA barcoding universal molecular genes were used to identify the species. Cytochrome b is a specific gene used for identification purpose. DNA barcoding is a reliable and effective method compared to the different traditional morphological methods of specie identification. So,in the present study which was conducted to identify the species, a total of 50 fish samples were collected from five different sites. DNA was extracted by using the Phenol Chloroform method from muscle tissue. Five sequences were sequenced (one from each site), analyzed, and identified specific species as Pangasius pangasius. Identified sequences were variable in length from 369 bp (Site 1), 364 bp (Site 2), 364 bp (Site 3), 352 bp (Site 4), and 334 bp (Site 5). Identity matches on the NCBI database confirmed the specific specie as P. pangasius. A distancing tree was drawn to show maximum likelihood among the same and different species. Yet, in many cases fishes on diverse development stages are difficult to identify by morphological characters. DNA-based identification methods offer an analytically powerful addition or even an alternative tool for species identification and phylogenetic study. This work intends to provide an updated and extensive overview on the DNA based methods for fish species identification by using Cytochrome b gene as targeted markers for identification purpose.

https://doi.org/10.1590/1519-6984.268001

Evaluation of methanolic crude extract of Linum usitatissimum for the removal of biofilm in diabetic foot isolates
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Haroon, M.

; Iqbal, M. J.

; Hassan, W.

; Ali, S.

; Ahmed, H.

; Hassan, S. U.

Brazilian Journal of Biology

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2023, Volume 83 elocation e245807

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Linum usitatissimum L é amplamente utilizado tradicionalmente para doenças múltiplas. O presente trabalho foi realizado para explorar a atividade antimicrobiana e antibiofilme do extrato bruto de Linum usitatissimum L (Lu.Cr). Foram realizadas análises fitoquímicas e aproximadas. As ataduras de pacientes diabéticos com pé foram recolhidas nos vários hospitais. As bandagens foram cultivadas para isolar as cepas bacterianas presentes nas mesmas. O método de difusão em disco foi utilizado para identificar o potencial antimicrobiano e a concentração inibitória mínima do Lu.Cr também foi determinada. A análise aproximada confirma o teor de umidade 8,33%, teor de cinzas 4,33%, proteína bruta 21,20%, gordura bruta 49,2% e fibra bruta 5,63%. Foi revelado que as bactérias Gram-positivas são mais prevalentes entre todos os grupos de estudo. Lu.Cr possui potencial bactericida significativo contra S. aureus entre todos os outros micróbios. Devido a esse potencial, as ligaduras revestidas com linhaça podem ser utilizadas alternativamente para o tratamento do pé diabético.

Abstract Linum usitatissimum L is a widely used traditionally for multiple ailments. The present research was carried out to explore the antimicrobial, and anti-biofilm activity of crude extract of Linum usitatissimum L (Lu. Cr). Phytochemical and proximate analyses were performed. The bandages of diabetic foot patients were collected from the various hospitals. The bandages were cultured to isolate the bacterial strains present on it. The disc diffusion method was used to identify the antimicrobial potential whereas the minimum inhibitory concentration of the Lu.Cr were also determined. Proximate analysis confirms moisture content 8.33%, ash content 4.33%, crude protein 21.20%, crude fat 49.2% and crude fiber 5.63%. It was revealed that Gram-positive bacteria are most prevalent among all study groups. Lu.Cr possess significant bactericidal potential against S. aureus among all other microbes. Owing to this potential, linseed coated bandages can be used alternatively for the treatment of diabetic foot.

https://doi.org/10.1590/1519-6984.245807 4 downloads

Reducing the colonial footprint through tutorials: a South African perspective on the decolonisation of education
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Hassan, S. L.

South African Journal of Higher Education

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Nov 2022, Volume 36 Nº 5 Páginas 77 - 97

Resumo: En | Texto: En | PDF: En

This article reports on how tutorials are employed as an instrument for the decolonisation of higher education and the promotion of inclusivity. The study was underpinned by activity theory and Bourdieu's social field theory. Since 2015, students have embarked on nationwide protests against a colonised higher education system that they lament also makes them feel unwelcome and alienated. They have demanded that tertiary institutions be inclusive, address social justice and apply decolonisation. The study reported in this article demonstrated that the tutorial field provided a nurturing space where black tutees were made to feel comfortable on account of the tutors' symbolic capital and habitus which they could relate to. It is argued here that pedagogical tools such as the application of tutees' mother-tongue (an example of decolonisation) and the use of group work has the potential to help integrate black tutees into academia. One needs to be cautious, however, that groups which are linguistically similar could be regarded as exclusive.

https://doi.org/10.20853/36-5-4325

Effect of acute heat stress on production performance and egg quality in four strains of chickens
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Ghoname, M.

; Elnaggar, A.

; Hassan, S.

; Habashy, W.

South African Journal of Animal Science

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2022, Volume 52 Nº 2 Páginas 168 - 176

Resumo: En | Texto: En | PDF: En

The objective of this study was to investigate differences between four strains of chickens on laying hen productivity and egg quality under acute heat stress (AHS). The study consisted of 44 hens from each of the Fayoumi (FY), Golden Sabahia (GS), White Leghorn (WL) and Lohman Brown (LB) strains that were 30 weeks old. They were allotted to two groups (a thermoneutral temperature regime (26.0 ± 1 °C) and the AHS regime (35 ± 1 °C) and 55 ± 5% relative humidity) for six hours. Egg number and feed intake were recorded. After imposition of the AHS, four hens per strain per treatment were randomly selected and slaughtered to calculate the relative mass of various organs and the number of follicles. Acute heat stress decreased feed intake of all strains significantly. There was a significant interaction between temperature treatment and strain on feed intake, egg weight, surface area of egg, yolk index, yolk weight, and albumen weight and on the relative weights of the various organs, except for the gizzard. Thus, the magnitude of the effects of AHS was strain dependent, with GS seemingly being less affected than the other strains.

https://doi.org/10.4314/sajas.v52i2.6 48 downloads

A new class of distributions as a finite functional mixture using functional weights
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BOUALI, DALAL LALA

; CHESNEAU, CHRISTOPHE

; SHARMA, VIKAS KUMAR

; BAKOUCH, HASSAN S.

Anais da Academia Brasileira de Ciências

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2021, Volume 93 Nº 2 elocation e20181019

Resumo: En | Texto: En | PDF: En

Abstract In this paper, we introduce a new family of distributions whose probability density function is defined as a weighted sum of two probability density functions; one is defined as a warped version of the other. We focus our attention on a special case based on the exponential distribution with three parameters, a dilation transformation and a weight with polynomial decay, leading to a new life-time distribution. The explicit expressions of the moments generating function, moments and quantile function of the proposed distribution are provided. For estimating the parameters, the method of maximum likelihood estimation is used. Two applications with practical data sets are given.

https://doi.org/10.1590/0001-3765202120181019 129 downloads

A weighted negative binomial Lindley distribution with applications to dispersed data
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BAKOUCH, HASSAN S

Anais da Academia Brasileira de Ciências

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Set 2018, Volume 90 Nº 3 Páginas 2617 - 2642

Resumo: En | Texto: En | PDF: En

Abstract A new discrete distribution is introduced. The distribution involves the negative binomial and size biased negative binomial distributions as sub-models among others and it is a weighted version of the two parameter discrete Lindley distribution. The distribution has various interesting properties, such as bathtub shape hazard function along with increasing/decreasing hazard rate, positive skewness, symmetric behavior, and over- and under-dispersion. Moreover, it is self decomposable and infinitely divisible, which makes the proposed distribution well suited for count data modeling. Other properties are investigated, including probability generating function, ordinary moments, factorial moments, negative moments and characterization. Estimation of the model parameters is investigated by the methods of moments and maximum likelihood, and a performance of the estimators is assessed by a simulation study. The credibility of the proposed distribution over the negative binomial, Poisson and generalized Poisson distributions is discussed based on some test statistics and four real data sets.

https://doi.org/10.1590/0001-3765201820170733 1273 downloads

Continuous Synthesis of a Green Fuel Additive Mixture with Highest Quantities of Solketalacetin and Solketal and Lowest Amount of Diacetin from Biodiesel-Derived Glycerol
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Gorji, Yadollah M.

; Ghaziaskar, Hassan S.

Journal of the Brazilian Chemical Society

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Jun 2018, Volume 29 Nº 6 Páginas 1218 - 1224

Resumo: En | Texto: En | PDF: En

This work presents a continuous, easy-to-scale-up system for glycerol conversion to a valuable fuel additive mixture such as solketalacetin ((2,2-dimethyl-1,3-dioxolan-4-yl)methyl acetate), solketal and minimum amount of diacetin with no byproducts. A two-stage reaction was conducted to synthesize the mixture. At first, glycerol was reacted with acetic acid in a continuous system to synthesize monoacetin using a small plug flow reactor and central composite design to optimize the variables related to monoacetin synthesis. Finally, an acetic acid:glycerol mole ratio of 3.7:1, a temperature of 79 °C, a flow rate of 0.9 mL min-1, and a pressure of 1 bar were determined as the optimum conditions. At the optimum condition, predicted and experimental yields of monoacetin were 63 and 62%, respectively. In the next stage, monoacetin and the residual glycerol were reacted with acetone to obtain a mixture of solketalacetin, solketal, and diacetin with the mole percentage of 62, 30, and 8%, respectively.

https://doi.org/10.21577/0103-5053.20170217 778 downloads

Differentiation of free-ranging chicken using discriminant analysis of phenotypic traits
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Al-Atiyat, Raed M.

; Aljumaah, Riyadh S.

; Abudabos, Alaeldein M.

; Alotybi, Masoud N.

; Harron, Raafat M.

; Algawaan, Abdulaziz S.

; Aljooan, Hassan S.

Revista Brasileira de Zootecnia

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Out 2017, Volume 46 Nº 10 Páginas 791 - 799

Resumo: En | Texto: En | PDF: En

ABSTRACT In this study, we investigated the differentiation of five different chicken ecotypes - Center, North, South, West, and East - of Saudi Arabia using discriminate analysis. The analysis was based on nine important morphological and phenotypic traits: body color, beak color, earlobe color, eye color, shank color, comb color, comb type, comb size, and feather distribution. There was a strong significant relationship between the phenotype and effect of geographic height in terms of comb type and earlobe color in males as well as body, beak, eye, and shank color. In particular, the comb type and earlobe color differentiated the ecotypes of males. Among the females, the beak, earlobe, eye, shank color, and feather distribution had more differentiating power. Moreover, the discriminant analysis revealed that the five ecotypes were grouped into three clusters; the Center and the North in one cluster, the West and the South ecotypes in the second for males, and the East ecotype in the last cluster. The female dendogram branching was similar to the male dendrogram branching, except that the Center ecotype was grouped with the North instead of the South. The East ecotype was highly discriminated from the other ecotypes. Nevertheless, the potential of recent individual migration between ecotypes was also noted. Accordingly, the results of the utilized traits in this study might be effective in characterization and conservation of the genetic resources of the Saudi chicken.

https://doi.org/10.1590/s1806-92902017001000001 1118 downloads

10.

QT interval prolongation associated with azithromycin/methadone combination
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Amer, S

; Hassan, S

; Shariffi, M

; Chueca, L

West Indian Medical Journal

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Dez 2013, Volume 62 Nº 9 Páginas 864 - 865

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

This report documents the occurrence of QT prolongation in a 57-year old man, on methadone replacement therapy, treated with azithromycin for community acquired pneumonia. This case highlights a hitherto unknown drug interaction. In light of ever-increasing use of azithromycin, it is imperative that azithromycin be used with caution in patients who are already on drugs that are known to cause QT prolongation or that cause torsades de pointes.

Este reporte documenta la ocurrencia de la prolongación del intervalo QT en un hombre de 57 años, en la terapia de reemplazo con metadona, tratado con azitromicina por pulmonía adquirida en la comunidad. Este caso destaca una interacción de medicamentos desconocida hasta ahora. En vista del uso cada vez mayor de la azitromicina, resulta absolutamente necesario usarla con precaución en pacientes que ya están bajo tratamiento con medicamentos de los cuales se sabe que causan prolongación del intervalo QT o que causan torsades de pointes.

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